Analysis of Mixtures with High Complexity by NMR
Identification and quantification of analytes in complex solution-state mixtures are critical procedures in many areas of Chemistry, Biology and Molecular Medicine. Nuclear magnetic resonance (NMR) is a unique tool for this purpose providing a wealth of atomic-detail information without requiring extensive fractionation of the samples. Our technology involves three new12-19 multidimensional-NMR based approaches that are geared toward the analysis of mixtures with high complexity at natural 13C abundance, including ones encountered into metabolomics. Common to all three approaches is the concept of the extraction of consensus 1D spectral traces or consensus 2D planes followed by clustering, which significantly improves the capability to identify mixture components that are affected by strong spectral overlap.