Device and Method for Concomitant Ejection and Suction of Perfusate
CESOP is a microfluidic device that enables focal application and clearance of drugs/compounds (perfusate) to nuclei or regions within acute brain slices submerged in artificial cerebrospinal fluid or other bathing media under non-laminar/ turbulent now conditions. The CESOP technique has distinct advantages over either bath perfusion or local perfusion which prove cumbersome and/or inadequate for studying how drug application to one region or the brain affects a neighboring (juxtaposed) region. The CESOP device/method enables rapid focal application of drugs/compounds while restricting their "spillover" to neighboring regions. Turbulent/non-laminar flow conditions that manifest in slice recording chambers exacerbate spillover thereby hindering/disrupting electrophysiological recordings and the study of region-specific drug effects. The CESOP device/method solves this problem through concomitant ejection and suction or perfusate, even under moderately turbulent conditions.