Dual-Chamber Perfusion Bioreactor for Orthopedic Tissue Interfaces
Adult mesenchymal stem cells (MSC's) have been used to regenerate bone and cartilage in both pre-clinical and clinical studies. This device can be used to fabricate from MSC's orthopedic interfaces such as bone-cartilage, ligament-bone, or muscle-tendon for implantation to correct orthopedic defects caused by disease or injury. The perfusion bioreactor chamber has two compartments connected by a porous scaffold for growing the tissue. The conditions such as substance concentration, pressure, and fluid flow rates can be individually controlled in each compartment and the pressure can be regulated so that the fluid can penetrate the scaffold transversely or horizontally. The porous scaffold supports cell growth and fluid penetration thereby providing the structure for the MSC's to form a functional tissue (bone, cartilage, etc.).
Large tissue constructs require a controlled heterogeneous environment to grow properly. Previous bioreactor technology typically creates a homogeneous growth environment by introducing media flow in one direction and is not able to control the communication between different regions of the construct.
- Able to control the biochemical and physiochemical conditions in each growth chamber individually
- Able to modulate the interactions and communication between two compartments by directing flow
- Enables the MSC's to differentiate two different cell types within the same construct; for example, chondrocytes and osteoblasts, thereby creating constructs composed of both cartilage and bone