Mesenchymal Stem Cells (MSC) Expansion
The present invention describes materials and methods for growing and expanding mammalian mesenchymal stem cells (MSC) while maintaining their undifferentiated phenotype, self-renewal ability, therapeutic potency, and/or multi-lineage potential. The method describes i) the seeding of freshly isolated MSC on a 3-D scaffold and their growth under physiological or low O2 tension for a period of time sufficient to support formation of 3-D extracellular matrix (ECM) network; ii) the decellularizing of the 3-D scaffold; and iii) the reseeding of the decellularized 3-D scaffold with MSCs, whereby the reseeded MSCs grow on the scaffold and maintain an undifferentiated phenotype. The 3-D scaffold comprises or is composed of poly(ethylene terephthalate) (PET).
A faster production of highly potent human MSC is obtained using our methodology based on combining hypoxia and cell-derived ECM compared with the traditional culture methods utilizing growth factor supplements and a high concentration of serum.
- Expands hMSC faster than the conventional methods
- Better presses hMSC's therapeutic potency compared with the traditional culture methods
- Requires a low concentration of serum and requires no exogenous factors to be added to expand the cells