Treatment of Lipid Encapsulated Surface Supported Drug Multilayers for In Vitro Delivery to Cells
Fluid Phospholipids have demonstrated their usefulness in biotechnology and biomimetic applications such as cell modeling and drug delivery. However, their exploitation has been limited to low throughput applications due to the instability of supported lipid multilayers to aqueous immersion. Our researchers have been successful in the immersion of supported lipid multilayers in simple buffers, immersion under high-protein-content media has remained a challenge.
Screening the effects of small molecules on cells grown in culture is a well-established method for drug discovery and testing, and faster throughput at lower cost is needed. Small molecule arrays and microfluidics are promising approaches. This technology consists of devices involving a microarray of lipid multilayers combined with silicon containing compounds to form a hybrid silicon based assembly on the surface of the lipid multilayer dot.
The devices can provide a surface mediated delivery of drugs to cells from the microarray of lipid multilayers encapsulating drug. The multilayer patterns can be of sub-cellular dimensions and controllable thickness and can be formed by dip-pen nanolithography. The patterns can successfully deliver a small molecule only to the cells directly over them, resulting in no cross-contamination to cells grown next to the patterns.
There is a need for lipid multilayer arrays that are stable in cell-based applications, stability of lipid multilayer arrays under high protein cell culture media, and for lipid multilayer arrays that allow for viable cell adhesion. Our researchers have developed lipid multilayer array devices which exhibit increased stability in cell-based applications such as high protein cull culture media, as well as allow for viable cell adhesion.